Study of Biphasic Aqueous Systems Formulated by Polyethylene Glycol and Tartarate Salts, and Purification of Lectin Conbol Obtained from Canavalia Boliviana
Name: ALEXANDRA CRISTINI GONÇALVES PINTO
Type: MSc dissertation
Publication date: 08/04/2016
Advisor:
Name |
Role |
|---|---|
| ANDERSON FUZER MESQUITA | Advisor * |
Examining board:
| Name |
Role |
|---|---|
| ANDERSON FUZER MESQUITA | Advisor * |
| DENISE ROCCO DE SENA | External Examiner * |
| ELOI ALVES DA SILVA FILHO | Internal Examiner * |
Summary: Purification of protein extracts is a complex process that involves several steps ranging
from protein precipitation by salt solutions to chromatographic separation methods. This
methodology, although well established in the scientific community, is high cost, high
uptime and finds it difficult to be expanded on an industrial scale . It is in this context that
the aqueous two-phase systems emerge as an economically viable and sustainable
alternative, from an environmental point of view.
For this study were developed two research fronts, divided in chapters 4 and 5 in this
work. At fourth one was evaluated the effect of temperature, polymer molecular weight
and the cation on the phase equilibria of the system formed by PEG3350 + potassium
tartrate + H2O, PEG10000 + potassium tartrate + H2O and PEG20000 + potassium tartrate
+ H2O in pH7 and 288K, 298K and 308K. . The results show that the increasing of
temperature, the increasing of polymers molecular weight and the cation of lower
hydration energy, shifted the bimodal toward the origin, WHERE is found lower values of
concentration of Polyethyle glycol (PEG) and salt.
In the fifth chapter we determined the partition coefficient (Kp), recovery rate (Y) and
purity (PHPLC) of ConBol protein in systems formed by PEG400 + KH2PO4 / K2HPO4 + H2O
+ PEG600 and KH2PO4 / K2HPO4 + H2O. With the results it is observed that the best
system to partition the ConBol protein is formed by PEG400 + KH2PO4 / K2HPO4 + H2O, in
concentrations of 16%, 18.8% and 65.2% respectively. This system showed a partition
coefficient (Kp) of 13.40, a recovery yeld (Y) of 6.42% and a purity of 69%.
